Plant DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Chidachi chimagwiritsa ntchito ndime ya DNA yokhayo yomwe imatha kumangirira DNA, Foregene protease ndi dongosolo lapadera la buffer, lomwe limathandizira kwambiri kuyeretsa kwa DNA ya zomera.DNA yapamwamba kwambiri ya genomic imatha kupezeka mkati mwa mphindi 30, zomwe zimapewa kuwonongeka kwa ma genomic DNA.
DNA-yokha silica gel nembanemba ntchito pa sipinamu ndime ndi Foregene latsopano zinthu zatsopano, amene mogwira ndi mwachindunji kumanga DNA, ndi kukulitsa kuchotsa RNA, zonyansa mapuloteni, ayoni, polysaccharides, polyphenols ndi zina organic mankhwala.
Zida zigawo
| Buffer PL1, Buffer PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Foregene Protease |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
■ Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zida limatheketsa kuchotsa RNA ku genomic DNA popanda RNase yowonjezera panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yakunja.
■ Liwiro lofulumira: Foregene Protease ili ndi zochita zambiri kuposa ma proteinase ofanana ndipo imagaya zitsanzo za minofu mofulumira.
■ Zosavuta: ntchito yochotsa ma genomic DNA imatha kutha mphindi 30.
■ Yabwino: The centrifugation ikuchitika kutentha firiji, palibe 4 ℃ otsika kutentha centrifugation kapena Mowa mpweya wa DNA chofunika.
■ Chitetezo: palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: DNA yoyeretsedwa ya genomic ili ndi tizidutswa tambiri, palibe RNA, palibe RNase, komanso ma ayoni otsika kwambiri, amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.
Kit application
Oyenera kuchotsa ndi kuyeretsa ma genomic DNA kuchokera kuzinthu zatsopano kapena zowuma.
Kuyenda kwa ntchito
Chithunzi
Kusungirako ndi moyo wa alumali
Zida zimatha kusungidwa kwa miyezi 12 kutentha kwapakati (15-25 ℃) ndi 2-8 ℃ kwa nthawi yayitali.
Njira ya Foregene Protease Plus ili ndi njira yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);ntchito yake ndi bata adzakhala bwino pamene kusungidwa pa4 ℃, kotero tikulimbikitsidwa kusunga pa 4 ℃, kumbukirani osati kusunga pa -20 ℃.
Vuto Kusanthula Buku
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Zokolola zochepa kapena palibe DNA
Nthawi zambiri pamakhala zinthu zambiri zomwe zimakhudza zokolola za DNA ya genomic, kuphatikiza komwe kumachokera chitsanzo, zaka zachitsanzo, momwe amasungiramo zitsanzo, komanso momwe amagwirira ntchito.
DNA ya Genomic sinapezeke pochotsa
1. Zitsanzo za minofu zimasungidwa molakwika kapena kusungidwa kwa nthawi yayitali, zomwe zimapangitsa kuti DNA ya genomic iwonongeke.
Malangizo: Sungani zitsanzo za minofu mu nayitrogeni wamadzimadzi kapena -20°C;yesani kugwiritsa ntchito zitsanzo zomwe zasonkhanitsidwa kumene pochotsa ma genomic DNA.
2. Zitsanzo zochepa kwambiri zitha kupangitsa kuti DNA yofananirayo isachotsedwe.
Lingaliro: Kwa zitsanzo za minofu zomwe zasungidwa kwa nthawi yayitali kapena zomwe zawonongeka kwambiri za DNA, kuchuluka kwa zitsanzo za minofu kumatha kuonjezedwa moyenera kuti mutulutse DNA yochuluka.Kuchuluka kwa chitsanzocho kungadziwike molingana ndi zosowa za DNA, koma chitsanzo chatsopano sichiyenera kupitirira 100mg, ndipo chitsanzo chouma sichiyenera kupitirira 30mg.
3. Chitsanzocho sichimadulidwa ndi nayitrogeni wamadzimadzi kapena kuikidwa kwa nthawi yayitali pambuyo pa nayitrogeni wamadzimadzi.
Lingaliro: Panthawi yochotsa DNA, chitsanzocho chiyenera kukhala chokwanira ndi nayitrogeni wamadzimadzi kuti aswe khoma la selo;mutatha kupera, chonde tumizani ufa wa chitsanzo ku PL1 wotenthedwa pa 65°C mwamsanga (pokhapokha ufa wa nthaka utasungunuka, DNA ya genomic idzayamba kuwonongeka mofulumira) .
4. Kusungirako kosayenera kwa Foregene Protease kumabweretsa ntchito yochepetsedwa kapena yosasinthika.
Malangizo: Tsimikizirani zosungirako za Foregene Protease kapena m'malo mwake ndi Foregene Protease yatsopano ya enzymatic hydrolysis.
5. Chidacho chimasungidwa molakwika kapena kusungidwa kwa nthawi yayitali kwambiri, zomwe zimapangitsa kuti zigawo zina za zidazo zilephereke.
Malangizo: Gulani zida zatsopano zopangira ma genomic DNA kuti zitheke.
6. Kugwiritsa ntchito zida molakwika.
Malingaliro: Gulani Plant DNA Isolation Kit yoperekedwa ku zitsanzo zochotsa ndi kuyeretsa DNA ya genomic DNA.
7. Buffer WB popanda kuwonjezera awopanda madzi ethanol.
Malangizo: Onetsetsani kuti mwawonjezera voliyumu yolondola ya ethanol ku Buffer WB.
8. Chosowacho sichinagwetsedwe pa nembanemba ya silika molondola.
Yesani: Onjezani zomwe zimatenthedwa kale pa 65℃dropwise pakati pa silika gel nembanemba, ndi kusiya firiji kwa mphindi 5 kuonjezera elution dzuwa.
M'zigawo kuti mupeze zokolola zochepa za genomic DNA
1. Chitsanzocho chimasungidwa molakwika kapena kusungidwa kwa nthawi yayitali, zomwe zimapangitsa kuti DNA ya genomic iwonongeke.
Malangizo: Sungani zitsanzo za minofu pa -20℃;yesetsani kugwiritsa ntchito zitsanzo za minofu zomwe zasonkhanitsidwa kumene pochotsa ma DNA a genomic.
2. Ngati kuchuluka kwa zitsanzo za minofu kuli kochepa kwambiri, DNA yotulutsidwa idzakhala yochepa.
Lingaliro: Zitsanzo za zomera zina zimakhala ndi madzi ambiri, monga zomera za m'madzi monga algae, ndi zina zotero, mlingo ukhoza kuonjezedwa moyenerera kapena madzi akhoza kukhala opanda madzi pang'ono opareshoni isanayambe.
3. Zitsanzozi sizinapangidwe bwino ndi nayitrogeni wamadzimadzi kapena zinasiyidwa kutentha kwanthawi yayitali pambuyo popera.
Ndemanga: Kukupera kwa nayitrogeni wamadzimadzi kuyenera kukhala kokwanira, ndipo khoma lachitsanzo la cell liyenera kuthyoledwa momwe kungathekere;atangomaliza kupera, chitsanzo cha ufa chiyenera kusamutsidwa ku 65℃preheated Buffer PL1 pa sitepe yotsatira.
4. Osagwiritsa ntchito zida zolondola.
Malangizo: Gwiritsani ntchito chida chodzipatula cha Plant DNA Isolation Kit kuchotsa ndi kuyeretsa DNA ya genomic ya mbewu.
5. Kusungirako kosayenera kwa Foregene Protease kumapangitsa kuti ntchito yochepetsedwa kapena yosasinthika.
Malangizo: Tsimikizirani zosungirako za Foregene Protease kapena m'malo mwake ndi Foregene Protease yatsopano ya enzymatic hydrolysis.
6. Vuto losalankhula
Malangizo: Chonde gwiritsani ntchito Buffer EB pakuwunikira;ngati mukugwiritsa ntchito ddH2O kapena zoyeserera zina, onetsetsani kuti pH ya zomwe zikuyenda bwino zili pakati pa 7.0-8.5.
7. Chosowacho sichinadonthedwe bwino
Yesani: Chonde onjezani dontho la elution pakati pa nembanemba ya silika ndikuisiya pa kutentha kwapakati kwa mphindi 5 kuti muwonjezere mphamvu ya elution.
8. Voliyumu yodziwika ndi yaying'ono kwambiri
Yesani: Chonde gwiritsani ntchito eluent pofotokozera za DNA molingana ndi malangizo, osachepera 100.μl.
DNA yotulutsidwa ya genomic yokhala ndi chiyero chochepa
Chiyero chochepa cha DNA ya genomic chidzatsogolera ku kulephereka kapena kuperewera kwa zoyesera zapansi, monga: enzyme siingadulidwe, ndipo gawo la jini lachindunji silingapezeke ndi PCR.
1. Kuwonongeka kwa mapuloteni osiyanasiyana, kuipitsidwa kwa RNA.
Kusanthula: Buffer PW sinagwiritsidwe ntchito kutsuka ndime;Buffer PW sinagwiritsidwe ntchito kutsuka ndime pa liwiro lolondola la centrifugation.
Lingaliro: yesetsani kuwonetsetsa kuti palibe mvula mumlengalenga pamene chowonjezera chikudutsa pamzati;onetsetsani kuti mwatsuka ndime yoyeretsera ndi Buffer PW molingana ndi malangizo, ndipo sitepe iyi siyingasiyidwe.
2. Kudetsedwa kwa ayoni.
Kusanthula: Gawo losamba la Buffer WB silinasiyidwe kapena kutsukidwa kamodzi kokha, zomwe zidapangitsa kuti pakhale kuipitsidwa kwa ionic.
Malangizo: Onetsetsani kuti mwasamba kawiri ndi Buffer WB molingana ndi malangizo kuti muchotse ayoni otsalira momwe mungathere.
3. Kuwonongeka kwa RNase.
Kusanthula: Exogenous RNase yawonjezedwa ku Buffer;Kuchapira kolakwika mu Buffer PW kupangitsa kuti pakhale RNase yotsalira ndikusokoneza machitidwe oyesera a RNA akumunsi, monga kulembera mu vitro.
Malingaliro: Foregene mndandanda nucleic asidi m'zigawo zida angathe kuchotsa RNA popanda RNase zina, ndi reagents onse Plant DNA Isolation Kit safuna RNase;onetsetsani kuti mwatsuka ndime yoyeretsera ndi Buffer PW molingana ndi malangizo, ndipo sitepe iyi siyingasiyidwe.
4. Zotsalira za Ethanol.
Kusanthula: Pambuyo kutsuka ndime yoyeretsa ndi Buffer WB, palibe chopanda kanthu chubu centrifugation chinachitidwa.
Malangizo: Tsatirani malangizo oyenera opanda kanthu chubu centrifugation.
Buku Lamalangizo:
Plant DNA Isolation Kit Instruction Manual
