China Chomera Mbewu(Polysaccharide Polyphenol wolemera, waung'ono) Direct PCR Plus Kit I-UNG(popanda Zitsanzo Zida) Wopanga ndi Wopereka |Foregene

Mbewu Zomera(Polysaccharide Polyphenol yolemera, yaying'ono) Direct PCR Plus Kit I-UNG(popanda Zida Zoyeserera)

Mbewu Zomera(Polysaccharide Polyphenol yolemera, yaying'ono) Direct PCR Plus Kit I-UNG(popanda Zitsanzo Zida) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Mwachindunji ntchito polysaccharide polyphenol mbewu lysate monga template kwa PCR matalikidwe, popanda m'zigawo osiyana DNA, kudya ndi mkulu tilinazo.Dongosolo la UNG loletsa kuwononga chilengedwe lawonjezedwa kuti tipewe zolakwika.

- Palibe kuyeretsa nthawi komanso mtengo wa DNA.

- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.

- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolera mwamphamvu kwa PCR reaction inhibitors.

- Anti-pollution PCR system 2 × Seed PCR EasyTM Mix (UNG), yomwe imatha kuthetsa kuipitsidwa ndi zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula mwamsanga DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi ma polysaccharides apamwamba ndi ma polyphenols (monga nthochi, thonje, ndi zina zotero) kuti zigwiritsidwe ntchito pazochitika za PCR, choncho ndizoyenera kwambiri kuyesa chibadwa chachikulu.Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, kwa zitsanzo za mbewu zomwe zimayenera kumera, mutha kusankha kudula zidutswa zazing'onoting'ono (1-5mg) kupatula miluza ya mbewu yoyesera;pazoyeserera zomwe zimafunikira kuyesa ndikuyesa zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).

Zofotokozera

50T,200T,500T,2000T

Zida zamagulu

Plant Seed Direct PCR Plus Kit I-UNG Polysaccharide Polyphenol Plant Seed (Yaing'ono) Direct PCR Kit-UNG
Kupanga kwa zida		Chithunzi cha TP-0316T	Mtengo wa TP-03161	Mtengo wa TP-03162	Mtengo wa TP-03163
Kupanga kwa zida		50 T	200 T	500 T	2000 T
Gawo I	Mtengo wa SSP1	4.5ml pa	18ml ku	45ml ku	90ml × 2
	Mtengo SP2	3ml ku	10 ml pa	25ml ku	100 ml
	6 × DNA Loading Buffer	1.5ml pa	1.5ml pa	1.5ml pa	1.5ml × 4
Gawo II	Mtengo wa SSP2	1.5ml pa	6ml ku	15ml ku	60 ml pa
Gawo II	2× Mbewu PCR EasyTM Mix	500μl	1mlx2 pa	1.7ml × 3	1.7ml × 12
Pamanja		1	1	1	1

Mbali ndi ubwino

- Palibe kuyeretsa nthawi komanso mtengo wa DNA.

- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.

- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

- Anti-kuipitsa PCR dongosolo 2 × Mbewu PCR Easy^TM Mix (UNG), yomwe imatha kuthetsa kuipitsidwa komwe kumachitika chifukwa cha zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.