China Bzalani Mbeu(Yaing'ono ndi Yapakatikati) Mwachindunji PCR Kit I-UNG(popanda Zitsanzo Zida) Wopanga ndi Wopereka |Foregene

Bzalani Mbewu(Yaing'ono ndi Yapakatikati) Direct PCR Kit I-UNG(popanda Zida Zoyesa Zitsanzo)

Bzalani Mbewu(Yaing'ono ndi Yapakatikati) Direct PCR Kit I-UNG(popanda Zida Zoyeserera) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Kugwiritsa ntchito mwachindunji kwa mbewu ya lysate ngati template yakukulitsa kwa PCR, palibe kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwambiri.Dongosolo la UNG loletsa kuwononga chilengedwe lawonjezedwa kuti tipewe zolakwika.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi polysaccharide yochepa ndi polyphenol (monga: mpunga, tirigu, fodya, chimanga, soya, etc.)Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, zitsanzo za mbeu zomwe zimafunikirabe kumera, mutha kusankha kudula mabala ang'onoang'ono (1-5mg) kupatulapo mluza wa mbewu kuti muyese;pazoyesera zomwe zimayenera kuyesedwa mu zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).

Zofotokozera

50T,200T,500T,2000T

Zida zamagulu

Bzalani Mbewu Direct PCR Kit I-UNG Bzalani Mbewu (Yaing'ono ndi Yapakatikati) Direct PCR Kit-UNG
Kupanga kwa zida		Mtengo wa TP-0312T	Mtengo wa TP-03121	Mtengo wa TP-03122	Mtengo wa TP-03123
Kupanga kwa zida		50 T	200 T	500 T	2000 T
Gawo I	Mtengo SP1	10 ml pa	40 ml pa	100 ml	400 ml
	Mtengo SP2	3ml ku	10 ml pa	25ml ku	100 ml
	6 × DNA Loading Buffer	1.5ml pa	1.5ml pa	1.5ml pa	1.5ml × 4
Gawo II	2 × Mbewu PCR Yosavuta^TMMix(UNG)	500μl	1mlx2 pa	1.7ml × 3	1.7ml × 12
Pamanja		1	1	1	1

Mbali ndi ubwino

- Palibe chifukwa chotengera nthawi komanso mtengo woyeretsa DNA umafunika.

- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.

- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolera mwamphamvu kwa PCR reaction inhibitors.

- Anti-kuipitsa PCR dongosolo 2 × Mbewu PCR Easy^TMMix (UNG) imatha kuthetsa kuipitsidwa komwe kumachitika chifukwa cha zinthu za PCR ndikuwonetsetsa kuti makulidwe ake ndi olondola.