RT-PCR Easyᵀᴹ I (Chigawo Chimodzi)
Kufotokozera Kwa Zida:
◮Chida cha sitepe imodzi chimathandizira kuti zolembera zosinthika ndi PCR zizichitika mu chubu chomwecho.Zimangofunika kuwonjezera template ya RNA, zoyambira zenizeni za PCR ndi RNase-Free ddH2O.
◮Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
Zofotokozera
Foregene One-Step RT-PCR EasyTM Zogulitsa zotsatizana zimazindikira zomwe zimachitika kuchokera ku RNA kupita ku DNA yokhala ndi zingwe ziwiri, ndiye kuti, zolembera zosinthika ndi kukulitsa kwa PCR zimamalizidwa munjira yomweyo ya centrifuge chubu ndi machitidwe omwewo, omwe amathandizira masitepe oyesera., zimakwaniritsatiye experimental pulogalamu, ndibwino kuyesa kuchita bwino.
RT-PCR YosavutaTM I(Chigawo chimodzi) ndi gawo limodzi la RT-PCR chida chopangidwa mwapadera ndi Foregene, chomwe chimazindikira kugwira ntchito mosalekeza kuchokera ku RT kupita ku PCR muchubu chomwecho.Opaleshoniyo ndi yosavuta komanso yachangu, yomwe imatha kuchepetsa kuipitsidwa panthawi yoyeserera.2×RT-PCR YosavutaTM Mix ili ndi kulolerana kwamphamvu, kukhazikika kwamafuta, kuchita bwino kwambiri komanso mwachindunjiizi kwa kukulitsa.
Zida zamagulu
| RT-PCR YosavutaTMIne (One Step) | ||
| Zamkatimu | RT-02011 | RT-02012 |
| 100T (20μl dongosolo) | 500T (20μl dongosolo) | |
| 2 × RT-PCR YosavutaTM Sakanizani | 1 ml | 1.7ml × 3 |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml × 3 |
| Buku Lolangiza | 1 chidutswa | 1 chidutswa |
Mbali & ubwino
- Chida cha sitepe imodzi chimathandizira kuti zolembera zosinthika ndi PCR zizichitika mu chubu chomwecho.Zimangofunika kuwonjezera template ya RNA, zoyambira zenizeni za PCR ndi RNase-Free ddH2O.
- Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
- Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcription reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
- Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
1.Mayendedwe
Njira yonse yoyendetsera kutentha kwa ayezi, kuonetsetsa kuti zida zili m'malo a <4 °C.
2. Zosungirako
Chidacho chimasungidwa pa -20 ° C.Sungani mankhwalawa mufiriji yokhazikika pa -20 ° C mutangolandira.Ngati zosungirako zili zoyenera,tmankhwala ake sanganyoze ntchito iliyonse panthawi yovomerezeka ya chaka chimodzi.
