RT-qPCR Easyᵀᴹ (Chigawo Chimodzi) -SYBR Green I
Kufotokozera Kwa Zida:
◮Chida cha gawo limodzi chimapanga zolembera zosinthika ndi qPCR machitidwe awiri mu chubu yomweyo, zimangofunika kuwonjezera template RNA, zoyambira za PCR ndi RNase-Free ddH.2O.
◮Zida zimatha kusanthula mwachangu komanso moyenera ma virus a RNA kapena kufufuza RNA.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
◮RT-qPCR YosavutaTM(One Step) -SYBR Green I kit imabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuthetsa zolakwika zakumbuyo ndi ma sign pakati pa zitsime, zomwe ndizosavuta kuti makasitomala azigwiritsa ntchito mitundu yosiyanasiyana ya zida za PCR.
Kufotokozera
RT-qPCR YosavutaTM (Chigawo Chimodzi) -SYBR Green I imagwiritsa ntchito dongosolo lapadera kuti liphatikize bwino machitidwe a RT ndi Real Time PCR, omwe amatha kumaliza msanga kudziwa kuchuluka kwa majini.Mankhwalawa ali ndi kulekerera kwamphamvu, kutsimikizika kwakukulu komanso kukhazikika.Zogulitsazo zili ndi kampani yapadera ya Foregene HotStar Taq DNA Polymerase, yomwe ili ndi maubwino okulitsa mwachangu (2Kb/min), kukulitsa bwino kwambiri, kuthekera kokulirapo kwamphamvu, komanso kutsika kosagwirizana poyerekeza ndi ma enzyme wamba a Taq.Amagwiritsidwa ntchito pano pa Real Time RT-PCR kuti achepetse kukulitsa kosakhazikika ndikuwongolera kulondola kwa PCR.
Zofotokozera
100 T (20μl dongosolo)
500 T (20μl dongosolo)
Zida zamagulu
| RT-qPCR YosavutaTM(Chigawo Chimodzi) -SYBR Green I | ||
| Zamkatimu Zamkati | Mtengo wa RT-02111 | Mtengo wa RT-02112 |
| 100T (20μl dongosolo) | 500T (20μl dongosolo) | |
| 2 × RT-qPCR YosavutaTM Mix-SYBR | 1 ml | 1.7ml × 3 |
| 50 × ROX Reference Dye | 200μl | 1 ml |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml × 3 |
| Buku Lolangiza | 1 chidutswa | 1 chidutswa |
Mbali & ubwino
- Kit ya gawo limodzi imapanga zolembera zosinthika ndi qPCR machitidwe awiri muchubu yomweyo, zimangofunika kuwonjezera RNA yachikale, zoyambira za PCR ndi RNase-Free ddH2O.
- Zida zimatha kusanthula mwachangu komanso moyenera ma virus a RNA kapena kutsata RNA.
- Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcription reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
- Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
- RT-qPCR YosavutaTM(One Step) -SYBR Green I kit imabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuthetsa zolakwika zakumbuyo ndi ma sign pakati pa zitsime, zomwe ndizosavuta kuti makasitomala azigwiritsa ntchito mitundu yosiyanasiyana ya zida za PCR.
Kayendedwe kantchito
Zosungirako
1.Mayendedwemikhalidwe
Njira yonse yamayendedwe otsika otsika oundana, kuonetsetsa kuti zida zili m'malo a <4 ℃.
2.Kusungirako mikhalidwe
Zida zimasungidwa pa -20 ℃.Kusunga mankhwala mu zonse kutentha firiji pa-20 ℃ atangolandira.Ngati zinthu zosungirako zili zoyenera, mankhwalawasichidzanyozetsa ntchito iliyonse pazaka zovomerezeka za 1.
