RT-PCR Easyᵀᴹ II(Magawo Awiri)
Kufotokozera Kwa Zida:
◮Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮ Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
Zofotokozera
RT-PCR YosavutaTM II(Njira ziwiri) ili ndi ma reagents onse a magawo awiri a RT-PCR, kuphatikiza machitidwe a RT ndi machitidwe a PCR mu zida zomwezo, ndikupereka kukhathamiritsa kwa RT Mix ndi PCR Mix.RT Mix yapadera imatha kupanga cDNA yapamwamba kwambiri yamtundu woyamba mosavuta komanso moyenera. 2× PCRZosavutaTM SakanizaniKuwonjezera ali ndi luso lapamwamba komanso kukweza kwapadera.Kuphatikizika kwachilengedwe kwa ziwirizi kumapangitsa kuzindikira kwa jini yomwe mukufuna kuti ikhale yovuta kwambiri.
Zida zamagulu
| RT-PCRZosavutaTM II (Njira ziwiri) | ||
| Zamkatimu | RT-02021 | RT-02022 |
| 100T(20μldongosolo) | 500T(20μldongosolo) | |
| 2 × RT YosavutaTM Sakanizani | 1ml | 1.7ml ×3 |
| 2× PCRZosavutaTM SakanizaniKuwonjezera | 1ml | 1.7ml ×3 |
| Mtundu woyamba (50μM) | 200μl | 1ml |
| Oligo(dT)18 Primer (50μM) | 200μl | 1 ml |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml pa |
| Buku Lolangiza | 1chidutswa | 1chidutswa |
Mbali & ubwino
-Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
-Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
-Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
1.Mayendedwe
Njira yonse yoyendetsera kutentha kwa ayezi, kuonetsetsa kuti zida zili m'malo a <4 °C.
2. Zosungirako
Chidacho chimasungidwa pa -20 ° C.Sungani mankhwalawa mufiriji yokhazikika pa -20 ° C mutangolandira.Ngati zosungirako zili zoyenera,tmankhwala ake sanganyoze ntchito iliyonse panthawi yovomerezeka ya chaka chimodzi.
