Foregene DNA Identification System 20A (Kutulutsa Kwaulere kwa DNA)
Kufotokozera Kwa Zida:
Dongosolo lozindikiritsa la Foregene DNA 20A limagwiritsa ntchito ukadaulo wamitundu isanu yolembera ma fulorosenti kukulitsa nthawi imodzi 19 STR loci ndi malo amodzi ogonana mu chubu chimodzi.Pamene tikukamba za 13 CODIS core loci, 20 core loci amakwaniritsa zofunikira za Ministry of Public Security za autosomal STR kit.Iye ndi wamanja wakumanja kwa forensic DNA database yomanga ndi chizindikiritso cha wachibale.
Kufotokozera
Dongosolo lozindikiritsa la Foregene DNA 20A limagwiritsa ntchito ukadaulo wamitundu isanu yolembera ma fulorosenti kukulitsa nthawi imodzi 19 STR loci ndi malo amodzi ogonana mu chubu chimodzi.Pamene tikukamba za 13 CODIS core loci, 20 core loci amakwaniritsa zofunikira za Ministry of Public Security za autosomal STR kit.Iye ndi wamanja wakumanja kwa forensic DNA database yomanga ndi chizindikiritso cha wachibale.
Zamkatimu
| Kit Compone | Dzina la Componet | Voliyumu (μl / chubu) | Kuchuluka (chubu) |
| PCR Pre-reaction reagent | 2.5 × PCR reaction buffer Ⅲ | 1000 | 2 |
| 5 × 20A kusakaniza koyambirira | 500 | 2 | |
| Kuwongolera DNA 9947A (1ng/μl) | 25 | 1 | |
| Deionized madzi | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Allelic ladder20A | 40 | 1 |
| Kulemera kwa molekyulu mkati mwa standardORG 500 | 150 | 2 | |
| Zindikirani | |||
Kusanthula Zambiri
1. Tsegulani pulogalamu ya ID ya Genemapper.Mukamagwiritsa ntchito izi kwa nthawi yoyamba, muyenera kulowetsa mapanelo, bin Set, Njira Yolingana ndi Kuchulukitsa, ndi 30, 30, 320, 320, 320, 390, 320, 390, 420, 420, 490, 500)
2. Lowetsani deta ya electrophoresis, sankhani magawo owunikira ofananirako monga gulu, njira yowunikira, ndi kukula kwake, ndikusintha mtundu wa chitsanzo cha Makwerero ku "Allelic Ladder" mu gawo la "Sample Type";yambani kusanthula deta.
Kusungirako Reagent
1. Mutalandira zida zozizira mu ayezi wouma kapena mapaketi a pulasitiki oundana, ngati sagwiritsidwa ntchito kwakanthawi, chonde sungani pansi -20 ° C kwa nthawi yayitali.
2. Chidacho chikatulutsidwa ndikugwiritsidwa ntchito, zidazo ziyenera kusungidwa pa 4 ° C musanayambe kuchitapo kanthu kuti musamazizira mobwerezabwereza ndi kusungunuka;Taq enzyme iyenera kusungidwa pa -20 ° C.
3. Pambuyo pochitapo kanthu, zida (kuphatikizapo kulemera kwa maselo mkati ndi makwerero) ziyenera kusungidwa mu "chipinda chodziwira electrophoresis" pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo pewani kukhudzana ndi zida zowonongeka kuti mupewe kuipitsidwa.
