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Foregene DNA Identification System 27Y

Foregene DNA Identification System 27Y Chithunzi Chowonetsedwa
  • Foregene DNA Identification System 27Y
  • Foregene DNA Identification System 27Y
  • Foregene DNA Identification System 27Y

Kufotokozera Kwa Zida:

Foregene DNA Identification System 27Y imagwiritsa ntchito luso lamakono la mitundu isanu ndi umodzi la fulorosenti kukulitsa 27 Y-STR loci nthawi imodzi.Kuphatikizirapo: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS5333, DYS39, DY39, DY39F38, DYS39, DY399 ​​0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Chidacho chimakhala ndi luso loletsa kuletsa, ndipo chimatha kukulitsa mwachindunji pepala losefera, khadi la FTA, swab ya thonje, khadi yamalovu, swab yapakamwa, ndipo itha kugwiritsidwanso ntchito kuchotsa ndi kuyeretsa ma tempulo a DNA kuzinthu zamakesi.

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Kufotokozera

Foregene DNA Identification System 27Y imagwiritsa ntchito luso lamakono la mitundu isanu ndi umodzi la fulorosenti kukulitsa 27 Y-STR loci nthawi imodzi.Kuphatikizirapo: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS5333, DYS39, DY39, DY39F38, DYS39, DY399 ​​0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Chidacho chimakhala ndi luso loletsa kuletsa, ndipo chimatha kukulitsa mwachindunji pepala losefera, khadi la FTA, swab ya thonje, khadi yamalovu, swab yapakamwa, ndipo itha kugwiritsidwanso ntchito kuchotsa ndi kuyeretsa ma tempulo a DNA kuzinthu zamakesi.

Zamkatimu

Zoyeserera za Reagent

Dzina lachigawo

Voliyumu (μl/chubu)

kuchuluka (chubu)

PCR pre-reaction kit

 2 × PCR Reaction Master Mix

1250

2
5 x 27Y Primer Mix

500

2
DNA Typing Standard 9948

20

2
Deionized madzi

1700

2

Post-PCR Reaction Kit

 27Y allele osakaniza Makwerero

20

2
Orange molecular kulemera mkati muyezo ORG500

150

2

Kusanthula Zambiri

1. Tsegulani pulogalamu ya ID ya Gene Mapper.Mukamagwiritsa ntchito izi kwa nthawi yoyamba, muyenera kulowetsa mapanelo, bin Set, Njira Yolingana ndi Kuchulukitsa, ndi 30, 30, 320, 38, 390, 390, 320, 420, 420, 49, 49, 48, 400).

2. Lowetsani deta ya electrophoresis, sankhani magawo owunikira ofananirako monga gulu, njira yowunikira, ndi kukula kwake, ndikusintha mtundu wa chitsanzo cha Makwerero ku "Allelic Ladder" mu gawo la "Sample Type";yambani kusanthula deta.

Kusungirako Reagent

1. Chonde sungani pansi -20 ° C (ngati sichikugwiritsidwa ntchito kwakanthawi), mutalandira zida zowuma mu ayezi wouma kapena mapaketi a ayezi a gel.

2. Chonde sungani zidazo pa 4 ° C musanayambe kuchitapo kanthu kuti mupewe kuzizira ndi kusungunuka mobwerezabwereza, zida zitatulutsidwa kuti zigwiritsidwe ntchito;enzyme iyenera kusungidwa pa -20 ° C.

2. Pambuyo pochitapo kanthu, zida (kuphatikizapo kulemera kwa maselo mkati ndi makwerero) ziyenera kusungidwa mu "chipinda chodziwira electrophoresis" pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo pewani kukhudzana ndi zida zowonongeka kuti mupewe kuipitsidwa.

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