Foregene DNA Identification System 30A (Yosachotsa)
Kufotokozera Kwa Zida:
FOREGENE DNA identification system 30A imagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi ya fluorescent kutikukula 29autosomalSTR gawo, imodziAmel locus ndi imodzi Yindel locus nthawi imodzi, ndipo akhoza mwachindunji kukulitsa fyuluta pepala, FTAmakadi, thonje swabs, makadi malovu,ndi mkunja swab.
Kufotokozera
FOREGENE DNA identification system 30A imagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi ya fluorescent kutikukula 29autosomalSTR gawo, imodziAmel locus ndi imodzi Yindel locus nthawi imodzi, ndipo akhoza mwachindunji kukulitsa fyuluta pepala, FTAmakadi, thonje swabs, makadi malovu,ndi mkunja swab.
Zamkatimu
Kukonzekera kwa Amplification System
Table 1: Kukonzekera kwapangidwe ka Standard (Zopanda zopanda) zokulitsa
|
| Cotsutsa | 25 μl dongosolo (μl) | 10 μl dongosolo (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30APrimer Mix | 5.0 | 2 | |
| Deionized madzi | 13.75 | 5.5 | |
| Bmutu banga | M'mimba mwake 1.2 mm | M'mimba mwake 1.2 mm | |
| Total reaction volume | 25 μl | 10 μl | |
Kusanthula Zambiri
Chithunzi chowonjezera 1:Mapu a Allelic Ladder Typing
Chithunzi chowonjezera 2:Kulemba kwa DNA muyeso wa 9948mapa
Kusungirako Reagent
1. Chonde sungani pansi -20 ° C, mutalandira zida zozizira mu ayezi wouma kapena mapaketi a ayezi a gel.
2. Chonde sungani gawo la pre-reaction chigawo 4 × premix VII pa -20 ° C, pambuyo pa zida zomwe zatulutsidwa kuti zigwiritsidwe ntchito, ndipo sungani ma reagents ena otsalawo pa 4 ° C kuti mupewe kuzizira mobwerezabwereza ndi kusungunuka.Ngati mlingo umodzi uli wochepa, ndi bwino kusunga pa -20 ° C pambuyo aliquoting,
3. Sungani zigawozo mutatha kuchitapo pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo musakhudze ma reagents musanayambe kuchitapo kanthu kuti mupewe kuipitsidwa.
