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Foregene DNA Identification System 30 AC (Mlandu)

Foregene DNA Identification System 30 AC (Mlandu) Chithunzi Chowonetsedwa
  • Foregene DNA Identification System 30 AC (Mlandu)
  • Foregene DNA Identification System 30 AC (Mlandu)
  • Foregene DNA Identification System 30 AC (Mlandu)
  • Foregene DNA Identification System 30 AC (Mlandu)

Kufotokozera Kwa Zida:

Foregene Chidziwitso cha DNA30A-C amagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi wolembera ma fulorosentikukula 29autosomalSTR loci, 1 Amel locus, 1 YIndelocus, ndi1 Malo oti anthu azigonana nawo, 2malo oyang'anira zamkati, omwe amagwiritsidwa ntchito kukulitsaDNAtemplate yochotsedwa ndikuyeretsedwa kuchokeraCzida izi.

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Kufotokozera

Foregene Chidziwitso cha DNA30A-C amagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi wolembera ma fulorosentikukula 29autosomalSTR loci, 1 Amel locus, 1 YIndelocus, ndi1 Malo oti anthu azigonana nawo, 2malo oyang'anira zamkati, omwe amagwiritsidwa ntchito kukulitsaDNAtemplate yochotsedwa ndikuyeretsedwa kuchokeraCzida izi.

Zamkatimu

Kukonzekera dongosolo la Amplification

Table 2: Kukonzekera Kukonzekera kwa Standard m'zigawo ndi kukulitsa dongosolo

 

Cotsutsa

25ul dongosolo (µl)

10μl dongosolo (μl)

Master Mix

 2.5 × PremixCⅡ

10.0

4.0
5 x 30 AC Primer Mix

5.0

2.0
Chithunzi cha DNA

2.5-10

1-4
Deionized madzi

Pangani ma reaction volume 25μl

Pangani kuchuluka kwa momwe mungachitire10 ml

Kusanthula Zambiri

Chithunzi chowonjezera 1:Mapu a Allelic Ladder Typing

Chithunzi chowonjezera 2:Kulemba kwa DNA muyeso wa 9948mapa

Kusungirako Reagent

1. Chonde sungani pansi -20 ° C, mutalandira zida zozizira mu ayezi wouma kapena mapaketi a ayezi a gel.

2. Chonde sungani gawo la pre-reaction chigawo cha 2.5 × premix CII pa -20 ° C pambuyo pochotsa zidazo kuti zigwiritsidwe ntchito, ndipo zotsalira zotsalira zowonongeka zimasungidwa pa 4 ° C kuti zisawonongeke mobwerezabwereza ndi kusungunuka.Ngati mlingo umodzi ndi wochepa, ndi bwino kusunga pa -20 ° C pambuyo aliquoting.

3. Sungani zigawozo mutatha kuchitapo pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo musakhudze ma reagents musanayambe kuchitapo kanthu kuti mupewe kuipitsidwa.

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