1. Chonde sungani pansi -20 ° C (Ngati simunagwiritse ntchito kwakanthawi), mutalandira zida zowumitsidwa mu ayezi wouma kapena mapaketi a ayezi a gel.

2. Chonde sungani gawo la pre-reaction chigawo 4 × premix VII pa -20 ° C, pambuyo pake zida zitatulutsidwa kuti zigwiritsidwe ntchito, ndipo zina zotsalira zowonongeka zimasungidwa pa 4 ° C kuti zisawonongeke mobwerezabwereza ndi kusungunuka.Ngati mlingo umodzi ndi wochepa, ndi bwino kusunga pa -20 ° C pambuyo aliquoting.

3. Sungani zigawozo mutatha kuchitapo pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo musakhudze ma reagents musanayambe kuchitapo kanthu kuti mupewe kuipitsidwa.