Foregene DNA Identification System 25A-C (Mlandu)
Kufotokozera Kwa Zida:
Foregene DNA Identification System 25A-C imagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi ya fluorescence kuti ikweze 23 autosomal STR loci, loci imodzi ya Amel, imodzi ya Yindel loci ndi malo awiri owongolera amkati amkati panthawi yokulitsa ma templates a DNA otengedwa ndikuyeretsedwa kuchokera ku zitsanzo.
Kufotokozera
Foregene DNA Identification System 25A-C imagwiritsa ntchito ukadaulo wamitundu isanu ndi umodzi ya fluorescence kuti ikweze 23 autosomal STR loci, loci imodzi ya Amel, imodzi ya Yindel loci ndi malo awiri owongolera amkati amkati panthawi yokulitsa ma templates a DNA otengedwa ndikuyeretsedwa kuchokera ku zitsanzo.
Zamkatimu
Kukonzekera dongosolo la Amplification
Kukonzekera Kukonzekera kwa Standard Extraction and Amplification System
| Zigawo | 10μl dongosolo (μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10 × 25A-CPrimer Mix | 1.0 | |
| Chithunzi cha DNA | 1—5 | |
| Deionized madzi | Pangani voliyumu ya 10μl |
Kusanthula Zambiri
Chithunzi chowonjezera 1:Makwerero a genotyping a allele
Chithunzi chowonjezera 2:Kulemba kwa DNA muyeso wa 9948mapa
Kusungirako Reagent
1. Mukalandira zida, chonde sungani pansi -20 ℃ ngati simugwiritsa ntchito panthawiyi.
2. Chidacho chikatulutsidwa ndikugwiritsidwa ntchito, chigawo cha pre-reaction chigawo cha PCR premixed solution chimasungidwa pa -20 ℃, ndipo zotsalira zotsalira zowonongeka zimasungidwa pa 4 ℃ kuti zisawonongeke mobwerezabwereza.Ngati mlingo umodzi uli wochepa, ndi bwino kuti usungidwe pa -20 ° C mutatha kulongedza.
3. Pambuyo pakuchitapo, zigawozo zimasungidwa pa 4 ℃ kuti zisawonongeke mobwerezabwereza.Osakhudza pre-reaction reagents kupewa kuipitsa.
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