1. Chonde zisungeni pansi pa -20 ° C (ngati sizinagwiritsidwe ntchito kwakanthawi), mutalandira zida zowumitsidwa mu ayezi wouma kapena mapaketi a ayezi a gel.

2. Chonde soreni pre-reaction component PCR master mix ndi -20 ° C, zida zitatulutsidwa kuti zigwiritsidwe ntchito ndipo zotsalira zotsalira zimasungidwa pa 4 ° C kuti zisazizira mobwerezabwereza ndi kusungunuka.Ngati mlingo umodzi ndi wochepa, ndi bwino kusunga pa -20 ° C pambuyo aliquoting.

3. Sungani zigawozo mutatha kuchitapo pa 4 ° C, pewani kuzizira mobwerezabwereza ndi kusungunuka, ndipo musakhudze ma reagents musanayambe kuchitapo kanthu kuti mupewe kuipitsidwa.