-
Matenda a Animal Direct PCR kit
Chida ichi chimagwiritsa ntchito chida chapadera cha lysis kuti amasule mwachangu ma genomic a DNA kuchokera ku mitundu yazinyama ya PCR, chifukwa chake ndioyenera kwambiri kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zofunika, ndipo DNA yomwe ingafalitsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2× PCR YosavutaTM Sakanizani muli ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo mutha kugwiritsa ntchito lysate yachitsanzo kuti ayesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi kukhazikika.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
