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Mbewa Mchira SuperDirect PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa ndi mbewa zamakutu kuti PCR ichitepo kanthu, motero ndioyenera makamaka kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer yatha mkati mwa mphindi 10-30 kutentha kwapakati (20-25 ° C). Palibe kutenthetsa komwe kumafunikira, ndipo njira zina monga kuchotsa mapuloteni ndi RNA sizofunikira. Zambiri zomwe zatulutsidwa za DNA zitha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × M-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
