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Lnc-RT Heroᵀᴹ I (Ndi gDNase) (Super Premix yoyamba-strand cDNA kaphatikizidwe kuchokera ku lncRNA)
Lnc-RT WopambanaTM Ine (Ndi gDNase) ndimachitidwe osinthira mosasinthika omwe apangidwa kuti lncRNA ichotse mwachangu kuwonongeka kwa genomic DNA. 5 × gDNase Mix itha kuchotsa mwachangu ma genome otsala mu RNA pa 42 ° C kwa mphindi 2, kupewa kupewa kusokonezedwa kwa genome pazotsatira za qPCR.
5 × L-RT WopambanaTM Sakanizani ili ndi Foregene LncRNA Reverse Transcriptase yopangidwa mwapadera ndi Foregene, womwe ndi mtundu watsopano wa reverse transcriptase makamaka wa lncRNA ndi ma tempuleti ena ovuta a RNA, okhala ndi RNA yolimba kwambiri komanso yosintha bwino kwambiri. Makina omwe adakonzedweratu amachititsa kuti kusinthaku kusinthidwe mwachangu ndipo amatha kusindikiza ma tempuleti a RNA okhala ndi GC yayikulu komanso kapangidwe kake kovuta. Chingwe choyambirira cha strand cDNA chitha kumalizidwa pa 42 ° C mumphindi 15.
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Nthawi Yeniyeni PCR Easyᵀᴹ-Taqman
2X Real PCR YosavutaTM Mix-Taqman woperekedwa ndi Real Time PCR EasyTM-Taqman kit ndi njira yatsopano ya premix yomwe imagwiritsa ntchito njira zowunikira za Real Time PCR zokukweza, zomwe zingathandize kwambiri kuti mankhwala azikhala achangu komanso omvera. ROX imaperekedwa ngati utoto wowongolera mkati.
2X Real PCR YosavutaTM Mix-Taqman ili ndi mawonekedwe oyamba a Foregene Taq DNA Polymerase. Poyerekeza ndi ma enzyme wamba a Taq, ili ndi maubwino okweza kwamphamvu kwambiri, kuthekera kwamphamvu kwamphamvu ndikutsika pang'ono. Ikhoza kuchepetsa kukweza kosakhala kwapadera ndikusintha kulondola kwa PCR.
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Nthawi Yeniyeni PCR Easyᵀᴹ-SYBR Green I kit
2X Real PCR YosavutaTM Sakanizani-SYBR yoperekedwa ndi Real Time PCR EasyTM-SYBR Green I kit ndi chida chatsopano chomwe chimagwiritsa ntchito SYBR Green I pakuwongolera kwamphamvu kwa Real Time PCR, komwe kumatha kukometsa kuzindikirika kwa zinthu ndi kuzindikira kwakanthawi. ROX imaperekedwa ngati utoto wowongolera mkati. Kukula kwa fluorescence kwa chida ichi ndi kasanu ndi katatu kuposa zinthu zomwezi, zomwe zimatha kukhala zowoneka bwino komanso zowonekera mozama pa template ya DNA.
2X Real PCR YosavutaTM Mix-SYBR ili ndi mawonekedwe oyamba otentha a Foregene Taq DNA Polymerase. Poyerekeza ndi ma enzyme wamba a Taq, ili ndi maubwino okweza kwamphamvu kwambiri, kuthekera kwamphamvu kwamphamvu ndikutsika pang'ono. Ikhoza kuchepetsa kukweza kosakhala kwapadera ndikusintha kulondola kwa PCR.
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PCR Heroᵀᴹ (Ndi Dye)
2X Real PCR YosavutaTM Sakanizani-SYBR yoperekedwa ndi Real Time PCR EasyTM-SYBR Green I kit ndi chida chatsopano chomwe chimagwiritsa ntchito SYBR Green I pakuwongolera kwamphamvu kwa Real Time PCR, komwe kumatha kukometsa kuzindikirika kwa zinthu ndi kuzindikira kwakanthawi. ROX imaperekedwa ngati utoto wowongolera mkati. Kukula kwa fluorescence kwa chida ichi ndi kasanu ndi katatu kuposa zinthu zomwezi, zomwe zimatha kukhala zowoneka bwino komanso zowonekera mozama pa template ya DNA.
2X Real PCR YosavutaTM Mix-SYBR ili ndi mawonekedwe oyamba otentha a Foregene Taq DNA Polymerase. Poyerekeza ndi ma enzyme wamba a Taq, ili ndi maubwino okweza kwamphamvu kwambiri, kuthekera kwamphamvu kwamphamvu ndikutsika pang'ono. Ikhoza kuchepetsa kukweza kosakhala kwapadera ndikusintha kulondola kwa PCR.
