-
Lnc-RT Heroᵀᴹ I (Ndi gDNase) (Super Premix yoyamba-strand cDNA kaphatikizidwe kuchokera ku lncRNA)
Lnc-RT WopambanaTM Ine (Ndi gDNase) ndimachitidwe osinthira mosasinthika omwe apangidwa kuti lncRNA ichotse mwachangu kuwonongeka kwa genomic DNA. 5 × gDNase Mix itha kuchotsa mwachangu ma genome otsala mu RNA pa 42 ° C kwa mphindi 2, kupewa kupewa kusokonezedwa kwa genome pazotsatira za qPCR.
5 × L-RT WopambanaTM Sakanizani ili ndi Foregene LncRNA Reverse Transcriptase yopangidwa mwapadera ndi Foregene, womwe ndi mtundu watsopano wa reverse transcriptase makamaka wa lncRNA ndi ma tempuleti ena ovuta a RNA, okhala ndi RNA yolimba kwambiri komanso yosintha bwino kwambiri. Makina omwe adakonzedweratu amachititsa kuti kusinthaku kusinthidwe mwachangu ndipo amatha kusindikiza ma tempuleti a RNA okhala ndi GC yayikulu komanso kapangidwe kake kovuta. Chingwe choyambirira cha strand cDNA chitha kumalizidwa pa 42 ° C mumphindi 15.
