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Mbidzi Nsomba Direct PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera yolumikizira lysis kutulutsa mwachangu genomic kuchokera ku zebrafish ndi ziwombankhanga zina zamadzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuyankha kwa PCR, kotero ndizoyenera makamaka kuyesa kwakukulu kwa majini. Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate ya nyemba kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer. Amagwiritsidwa ntchito molumikizana ndi lysis buffer amatha kuzindikira mosavuta zitsanzo, ndipo ali ndi mawonekedwe a chidwi chachikulu, kulimba kwamphamvu, ndi kukhazikika kwabwino.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
