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Matenda a Animal Direct PCR kit
Chida ichi chimagwiritsa ntchito chida chapadera cha lysis kuti amasule mwachangu ma genomic a DNA kuchokera ku mitundu yazinyama ya PCR, chifukwa chake ndioyenera kwambiri kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zofunika, ndipo DNA yomwe ingafalitsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2× PCR YosavutaTM Sakanizani muli ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo mutha kugwiritsa ntchito lysate yachitsanzo kuti ayesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi kukhazikika.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Mbewa Mchira SuperDirect PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa ndi mbewa zamakutu kuti PCR ichitepo kanthu, motero ndioyenera makamaka kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer yatha mkati mwa mphindi 10-30 kutentha kwapakati (20-25 ° C). Palibe kutenthetsa komwe kumafunikira, ndipo njira zina monga kuchotsa mapuloteni ndi RNA sizofunikira. Zambiri zomwe zatulutsidwa za DNA zitha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × M-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Zilonda Zanyama Direct PCR kit-UNG
Chida ichi chimagwiritsa ntchito chida chapadera cha lysis kuti amasule mwachangu ma genomic a DNA kuchokera ku mitundu yazinyama ya PCR, chifukwa chake ndioyenera kwambiri kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zofunika, ndipo DNA yomwe ingafalitsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2×PCR YosavutaTM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2×PCR YosavutaTM Sakanizani, ndikuwonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi tanthauzo lililonse pazomwe mulibe uracil, potero kuwonetsetsa kuti kulimbitsa ndikulondola ndikulondola ndikuletsa kuthekera kwa kuipitsidwa kwa zinthu za PCR pakuyesa kwamitundu yayikulu.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Mbewa Mchira Direct PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa, makutu, minofu ndi mitundu ina ya minyewa yamachitidwe a PCR, chifukwa chake ndioyenera makamaka kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × M1-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Mbewa Mchira Direct PCR zida-UNG
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa, makutu, minofu ndi mitundu ina ya minyewa yamachitidwe a PCR, chifukwa chake ndioyenera makamaka kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × M1-PCR Easy TM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2 × M1-PCR Easy TM Mix (UNG), ndipo imawonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP ku nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi zotsatirapo pazomwe sizikhala ndi uracil, potero zimatsimikizira kulondola ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesedwa kwamitundu yayikulu. Kuwonongeka kwa zinthu za PCR kunachitika.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Mbidzi Nsomba Direct PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera yolumikizira lysis kutulutsa mwachangu genomic kuchokera ku zebrafish ndi ziwombankhanga zina zamadzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuyankha kwa PCR, kotero ndizoyenera makamaka kuyesa kwakukulu kwa majini. Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate ya nyemba kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer. Amagwiritsidwa ntchito molumikizana ndi lysis buffer amatha kuzindikira mosavuta zitsanzo, ndipo ali ndi mawonekedwe a chidwi chachikulu, kulimba kwamphamvu, ndi kukhazikika kwabwino.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
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Mbidzi Nsomba Direct PCR Kit-UNG
Chida ichi chimagwiritsa ntchito njira yapadera yolumikizira lysis kutulutsa mwachangu genomic kuchokera ku zebrafish ndi ziwombankhanga zina zamadzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuyankha kwa PCR, kotero ndizoyenera makamaka kuyesa kwakukulu kwa majini. Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × PCR Easy TM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2 × PCR Easy TM Mix, ndipo imawonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi zotsatirapo pazomwe sizikhala ndi uracil, potero zimatsimikizira kulondola ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesedwa kwamitundu yayikulu. Kuwonongeka kwa zinthu za PCR kunachitika.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
